Updated to 30,561 COVID-19 WGS from GISAID.
Table 1. Results from PSET analysis. The five Noblis assays were compared alongside the four assays from Corman and three assays from the CDC. Along with assays from China, Hong Kong, Thailand, Japan, and France. Each assay was tested using SARS-CoV-2 as the intended target. All off-target hits (TN, PF, FP) are to entries in NCBI BLAST databases (nt, gss, and env_nt).
| identifier | type | PT | TP | FN | Percent True | PF | FP | TN |
|---|---|---|---|---|---|---|---|---|
| Noblis.12 | Probe | 30281 | 139 | 141 | 99.54% | 0 | 0 | 680 |
| Noblis.40 | Probe | 29454 | 1013 | 94 | 99.69% | 0 | 1 | 1420 |
| Noblis.42 | Probe | 30352 | 60 | 149 | 99.51% | 0 | 2 | 1136 |
| Noblis.44 | Probe | 30013 | 95 | 453 | 98.52% | 0 | 1 | 1786 |
| Noblis.57 | Probe | 30033 | 267 | 261 | 99.15% | 0 | 1 | 853 |
| ncov_e_gene | Probe | 30355 | 81 | 125 | 99.59% | 361 | 15 | 53 |
| ncov_n_gene | Probe | 30153 | 266 | 142 | 99.54% | 0 | 340 | 72 |
| ncov_rdrp_1 | Probe | 0 | 30483 | 78 | 99.74% | 433 | 105 | 5967 |
| ncov_rdrp_2 | Probe | 2 | 30483 | 76 | 99.75% | 2 | 73 | 5916 |
| cdc_n1 | Probe | 29815 | 664 | 82 | 99.73% | 0 | 2 | 381 |
| cdc_n2 | Probe | 29912 | 249 | 400 | 98.69% | 0 | 1 | 372 |
| cdc_n3 | Probe | 29996 | 497 | 68 | 99.78% | 1 | 353 | 36 |
| China_N | Probe | 22004 | 375 | 8182 | 73.23% | 0 | 2 | 691 |
| China_ORF1ab | Probe | 28846 | 183 | 1532 | 94.99% | 1 | 0 | 367 |
| France_nCoV_IP2 | Probe | 30343 | 122 | 96 | 99.69% | 1 | 0 | 367 |
| France_nCoV_IP4 | Probe | 30271 | 208 | 82 | 99.73% | 0 | 0 | 1190 |
| HKU-N | Probe | 29953 | 218 | 390 | 98.72% | 327 | 33 | 34 |
| HKU-ORF1b-nsp14 | Probe | 30247 | 209 | 105 | 99.66% | 324 | 29 | 452 |
| Japan_NIID_2019-nCOV_N | Probe | 0 | 30160 | 401 | 98.69% | 0 | 0 | 384 |
| Japan_NIID_WH-1_F24381 | Outer | 30007 | 394 | 160 | 99.48% | 0 | 1 | 601 |
| Japan_NIID_WH-1_F501 | Outer | 29912 | 437 | 212 | 99.31% | 0 | 1 | 370 |
| Japan_NIID_WH-1_F509 | Outer | 30075 | 265 | 221 | 99.28% | 1 | 0 | 369 |
| Japan_NIID_WH-1_Seq_F24383 | Outer | 30002 | 391 | 168 | 99.45% | 0 | 0 | 606 |
| Japan_NIID_WH-1_Seq_F519 | Outer | 29898 | 320 | 343 | 98.88% | 0 | 1 | 369 |
| Japan_WuhanCoV-spk1 | Outer | 30039 | 352 | 170 | 99.44% | 1 | 1 | 594 |
| Thailand_WH-NIC_N | Probe | 30203 | 273 | 85 | 99.72% | 0 | 2 | 375 |