Preliminary in silico assessment of the specificity of published molecular assays and design of new assays using the available whole genome sequences of 2019-nCoV

Updated to 771,942 COVID-19 WGS from GISAID.

Table 1. Results from PSET analysis. The five Noblis assays were compared alongside the four assays from Corman and three assays from the CDC. Along with assays from China, Hong Kong, Thailand, Japan, France, South Korea, and Singapore. Each assay was tested using SARS-CoV-2 as the intended target. All off-target hits (TN, PF, FP) are to entries in NCBI BLAST databases (nt and env_nt). *edited 2/2 to include 5 additional assays.

Row Labels PT TP FN Percent True PF FP TN
Noblis.12 751902 16270 3770 99.51% 0 0 680
Noblis.40 759303 10714 1925 99.75% 0 1 1420
Noblis.42 759957 6846 5139 99.33% 0 2 1136
Noblis.44 757518 7538 6886 99.11% 0 1 1786
Noblis.57 745115 9858 16969 97.80% 0 1 853
ncov_e_gene 763494 2982 5466 99.29% 361 15 53
ncov_n_gene 742875 21376 7691 99.00% 0 340 72
ncov_rdrp_1 1 758766 13175 98.29% 433 105 5967
ncov_rdrp_2 18 759274 12650 98.36% 2 73 5916
cdc_n1 737065 27906 6971 99.10% 0 2 381
cdc_n2 738615 25089 8238 98.93% 0 1 372
cdc_n3 737539 26645 7758 99.00% 1 353 36
China_N 377301 67268 327373 57.59% 0 2 691
China_ORF1ab 743986 7491 20465 97.35% 1 0 367
France_nCoV_IP2 753238 8580 10124 98.69% 1 0 367
France_nCoV_IP4 753143 16135 2664 99.65% 0 0 1190
HKU-N 741887 21156 8899 98.85% 327 33 34
HKU-ORF1b-nsp14 759029 6634 6279 99.19% 324 29 452
Japan_NIID_2019-nCOV_N 0 763112 8830 98.86% 0 0 384
Japan_NIID_WH-1_F24381 746657 11058 14227 98.16% 0 1 601
Japan_NIID_WH-1_F501 758595 7816 5531 99.28% 0 1 370
Japan_NIID_WH-1_F509 750562 15625 5755 99.25% 1 0 369
Japan_NIID_WH-1_Seq_F24383 746457 11174 14311 98.15% 0 0 606
Japan_NIID_WH-1_Seq_F519 747842 10190 13910 98.20% 0 1 369
Japan_WuhanCoV-spk1 750347 7330 14265 98.15% 1 1 594
Thailand_WH-NIC_N 749358 15508 7076 99.08% 0 2 375
Chan-N 740611 22101 9230 98.80% 4 3 353
Chan-ORF1ab 29 762175 9738 98.74% 0 5 2
Chan-S 748319 11443 12180 98.42% 5 0 2
Niu-E 757456 8908 5578 99.28% 26 359 0
Won-E 7 766909 5026 99.35% 0 384 1
Won-N 747979 16776 7187 99.07% 8 7 358
Won-ORF1ab 753654 4908 13380 98.27% 5 2 313
Won-S 741106 6212 24624 96.81% 5 1 0
Yip-ORF1ab 756396 6385 9161 98.81% 5 1 1
Young-N 1 764112 7829 98.99% 0 5 1
Young-ORF1ab 756776 11889 3277 99.58% 5 1 0
Young-S 549744 31094 191104 75.24% 4 1 0
C1_COV_ORF1a 738462 13273 20207 97.38% 0 0 0
C2_COV_ORF1b 744087 20333 7522 99.03% 0 0 0
C3_COV_ORF3a 657954 92924 21064 97.27% 0 0 0
C4_COV_ORF8 561543 191450 18949 97.55% 0 0 0
C5_COV_S_gene 747066 11032 13844 98.21% 0 0 0