Preliminary in silico assessment of the specificity of published molecular assays and design of new assays using the available whole genome sequences of 2019-nCoV

Updated to 669,911 COVID-19 WGS from GISAID.

Table 1. Results from PSET analysis. The five Noblis assays were compared alongside the four assays from Corman and three assays from the CDC. Along with assays from China, Hong Kong, Thailand, Japan, France, South Korea, and Singapore. Each assay was tested using SARS-CoV-2 as the intended target. All off-target hits (TN, PF, FP) are to entries in NCBI BLAST databases (nt and env_nt). *edited 2/2 to include 5 additional assays.

Row Labels PT TP FN Percent True PF FP TN
Noblis.12 652737 14167 3007 99.55% 0 0 680
Noblis.40 659076 9152 1683 99.75% 0 1 1420
Noblis.42 660034 5746 4131 99.38% 0 2 1136
Noblis.44 657855 6362 5694 99.15% 0 1 1786
Noblis.57 648798 8136 12977 98.06% 0 1 853
ncov_e_gene 662714 2536 4661 99.30% 361 15 53
ncov_n_gene 644728 18601 6582 99.02% 0 340 72
ncov_rdrp_1 1 660834 9076 98.65% 433 105 5967
ncov_rdrp_2 17 661222 8672 98.71% 2 73 5916
cdc_n1 641063 23804 5044 99.25% 0 2 381
cdc_n2 641469 21187 7255 98.92% 0 1 372
cdc_n3 641024 23232 5655 99.16% 1 353 36
China_N 337390 53714 278807 58.38% 0 2 691
China_ORF1ab 647227 5924 16760 97.50% 1 0 367
France_nCoV_IP2 655931 6583 7397 98.90% 1 0 367
France_nCoV_IP4 655759 11939 2213 99.67% 0 0 1190
HKU-N 644999 17401 7511 98.88% 327 33 34
HKU-ORF1b-nsp14 659881 5560 4470 99.33% 324 29 452
Japan_NIID_2019-nCOV_N 0 662510 7401 98.90% 0 0 384
Japan_NIID_WH-1_F24381 650617 8728 10566 98.42% 0 1 601
Japan_NIID_WH-1_F501 658512 6520 4879 99.27% 0 1 370
Japan_NIID_WH-1_F509 651610 13233 5068 99.24% 1 0 369
Japan_NIID_WH-1_Seq_F24383 650396 8886 10629 98.41% 0 0 606
Japan_NIID_WH-1_Seq_F519 648904 8640 12367 98.15% 0 1 369
Japan_WuhanCoV-spk1 653563 5734 10614 98.42% 1 1 594
Thailand_WH-NIC_N 651646 13129 5136 99.23% 0 2 375
Chan-N 643184 18978 7749 98.84% 4 3 353
Chan-ORF1ab 24 661441 8446 98.74% 0 5 2
Chan-S 650869 8827 10215 98.48% 5 0 2
Niu-E 657851 7272 4788 99.29% 26 359 0
Won-E 7 665621 4283 99.36% 0 384 1
Won-N 649249 14520 6142 99.08% 8 7 358
Won-ORF1ab 656774 4244 8893 98.67% 5 2 313
Won-S 643506 4541 21864 96.74% 5 1 0
Yip-ORF1ab 656804 5396 7711 98.85% 5 1 1
Young-N 1 664199 5711 99.15% 0 5 1
Young-ORF1ab 656255 10854 2802 99.58% 5 1 0
Young-S 495902 24739 149270 77.72% 4 1 0
C1_COV_ORF1a 640819 10816 18276 97.27% 0 0 0
C2_COV_ORF1b 647087 17247 5577 99.17% 0 0 0
C3_COV_ORF3a 578321 73005 18585 97.23% 0 0 0
C4_COV_ORF8 504638 150508 14765 97.80% 0 0 0
C5_COV_S_gene 650533 9356 10022 98.50% 0 0 0