The African Center of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemers University Nigeria (Ede, Osun State, Nigeria), in collaboration with two partner institutions in Nigeria (the Nigerian Center for Disease Control, Abuja, Nigeria, and the Virology Unit, Central Research Laboratory, College of Medicine, University of Lagos, Lagos State, Nigeria) as well as the Viral Hemorrhagic Fever Consortium, is releasing Ebola virus (EBOV) genomes from thirteen cases from the 2014 EVD outbreak in Nigeria.
The sequences can be downloaded here:
EBOV_NGA_combined.fasta.txt (242.8 KB – this is a fasta file ending in “.txt” because virological only allows a specific set of filename extensions to be attached)
Samples were processed and extracted and Nextera libraries were constructed by ACEGID/Redeemers University staff using protocols described in Gire, et al (2014) and Matranga, et al (2014). Sequences were generated by 101bp PE Illumina sequencing on MiSeq and HiSeq machines at the Broad Institute. Genomes were assembled using the software pipeline described in Park, et al (2015).
We are in the process of gathering metadata, but at this moment we have not finalized the dates or locations, which is why we haven’t yet posted the data to NCBI. The total duration of the Nigerian EVD epidemic was relatively short however. All cases are from Lagos, except for one that is from Port-Harcourt (DHB_015_09_14). The index case that entered the country from Liberia is DHB_001_07_14.
Please feel free to download, share, use, and analyze this data. We are currently in the process of preparing a publication and will post progress on this forum. If you intend to use these sequences for publication prior to the release of our paper, please contact us directly. If you are interested in joining our collaboration–or if you have any other questions–then please also contact us directly.