Please use BioProject link instead since the sequences have all be finalized:
As part of our continued collaboration with the Sierra Leone Ministry of Health and Sanitation, Kenema Government Hospital and VHFC, a couple of weeks ago we received a new batch of inactivated EBOV samples from Sierra Leone for sequencing at the Broad Institute. Thirty-six hours ago our first run completed and we have now assembled 21 full-length genomes covering dates in June and October/early November. The assembled consensus sequences can be downloaded here:
New link (45 sequences):
The sequences were generated by 101bp PE Illumina sequencing using the protocols described in the Gire et al. and Matranga et al. papers. The genomes were assembled using Trinity, followed by an alignment refinement step with NovoAlign. The average bp coverage for this dataset was 1,056x [29x - 9,555x].
We have more samples and expect to be releasing data over the coming weeks and months - this was our initial run on this batch of samples. We are in the process of gathering metadata, but at this moment we don’t have any exact dates or other metadate for the individual samples.
We are currently in the process of preparing the data for GenBank and SRA submissions. Please note that this is an early release, so accuracy can’t be guaranteed at this stage.
Please feel free to download, share, use, and analyze this data. We are currently in the process of preparing a publication and will post progress on this forum. If you intend to use these sequences for publication prior to the release of our paper, please contact us directly.